UGM 2001 Uji Toksisitas Akut

Aim of study

To test the potential acute toxicity of capsules Chang Sheuw Tian Ran Ling Yao expressed as LD50 dose.
Mode of death during pre-clinical testing.
Understanding the spectrum of toxic effects (if any)

Research Team

Dr. Imono Argo Donatus, SU., Apt
Arief Nurrochmad, S.Si., Apt
Arief Rahman Hakim, S.Si., Apt
drh. Retno Murwant

Test result

NUMBER OF RATS THAT DIE (%) RESPONSE 14 DAYS AFTER ORAL ADMINISTRATION OF CHANG SHEUW TIAN RAN LING YAO CAPSULE SUSPENSION

Evaluation of acute and subacute toxicity of extract Chang Sheuw Tian Ran Ling Yao in mice Tabel 2

Table 1: Examination results of Acute toxicity Potency (death response) based on administration of dose (LD50).

Up to the 14th day post-administration of the Chang Sheuw Tian Ra Ling Yao Capsule suspension by oral at a dose of 20-12500 mg/Kg BW, no death of male or female mice was recorded (% Response = 0).

Gross description of pathology and several important organs of male rats on the 14th day after administration of a single dose by oral suspension of Chang Sheuw Tian Ran Ling Yao Capsules

Evaluation of acute and subacute toxicity of extract Chang Sheuw Tian Ran Ling Yao in mice Tabel 1

Table 2: Examination results of Acute Toxicity Potency towards vital organs based on administration of dose (LD50).

Results Summary

Extract Chang Sheuw Tian Ran Ling Yao on doses of 20 – 12,500 mg/kg did not reveal any signs of toxicity or mortality in any animal, during the 14 days observation period. No death was recorded (% response = 0).
The maximum dosing of extract Chang Sheuw Tian Ran Ling Yao (12,500 mg/kg BW), does not cause death.
The gross pathological examination results up to a dose of 12,500 mg/kgBW did not show any impairment in the vital organs of mice.
The results of this study suggest that extract Chang Sheuw Tian Ran Ling Yao, does not show any significant spectrum of toxic effects, Hence, should be considered as non-toxic and safe for medicinal use.

Conclusion

In conclusion, the acute toxicity study on extract Chang Sheuw Tian Ran Ling Yao suggests that a dose up to 12,500mg/kg BW (Highest variable) is devoid of any adverse effects in mice. Hence, this study suggests that this study suggest that extract Chang Sheuw Tian Ran Ling Yao should be considered as non-toxic and safe for medicinal use.