UGM 2002 Uji Efek Penghambatan Karsinogenesis Pada Tikus

Effect of Extract Chang Sheuw Tian Ran Ling Yao (CSTRLY) on BENZO[a]PYRENE Induced Lung Carcinogenesis in the Initiation and Post Initiation Phases in Mice

Aim

To determine the effect of Chang Sheuw Tian Ran Ling Yao (CSTRLY) extract on the inhibition of lung tumour growth in initiation and post initiation phases in mice induced by benzo(a)pyrene (B[a]P) and dimethylbenz(a) anthracene (DMBA).

Method

This study was conducted using the Newborn Mice method using Balpc strain mice (Mus Musculus). Newborn mice were divided into eight groups, each consisting of 10-15 mice. The first group was injected with B[a]P dissolved in dimethylsulfoxide (DMSO) intraperitoneally on the 1st, 8th, and 15th day after birth with doses of 0.2µmol, 0.4µmol, and 0.8µmol respectively.

Research Team

Prof. Dr. Ahmad Fudholi, D.E.A., Apt
Drs. Edy Meiyanto, M. Si., PHD, Apt
Drh. Retno Murwanati
Ganti Winarno Putra
Andi Eviyanti. T

Test Results

Results Summary

The Administration of extract Chang Sheuw Tian Ran Ling Yao once every 3 days for 30 days after birth revealed reduction on the percentage of mice affected by tumours, and the percentage of tumour growth in the lungs of mice induced by the carcinogen benzo(a)pyrene.

Initiation Phase

Extract with Dose I (450 mg/kg BW) revealed a reduction of tumour growth by 50.47%

Extract with Dose I (450 mg/kg BW) revealed a reduction of tumour growth by 79.22%

Extract with Dose II (750 mg/kg BW) revealed a reduction of tumour growth by by 57.03%

Post-Initiation Phase

Extract with Dose II (750 mg/kg BW) revealed a reduction of tumour growth by 41.40%

Extract with Dose III (1500 mg/kg BW) evealed a reduction of tumour growth by 80.46%

Extract with Dose III (1500 mg/kg BW) revealed a reduction of tumour growth by 76.56%

Conclusion

This study revealed that the herbal extract of Chang Sheuw Tian Ran Ling Yao conveyed the potential to reduce the incidence of tumours induced by carcinogen administration, both B[a]P and DMBA. Moreover, extract Chang Sheuw Tian Ran Ling Yao is also revealed the inhibition of the development of lung carcinogenesis in mice, especially in the early post-initiation phase.