Dharmais 2006 Uji Efektifitas Secara Invitro (cell line)

Aim

To investigate in-vitro, the potential of carcinogenesis inhibition through cytotoxic and apoptotic activity (cell line) on 4 types of cancer cells:

Cervical Cancer Cells (CaSki cell line)
Colon Cancer Cells (HT-29 cell line)
Breast Cancer Cells (MCF-7)
Cells Oral Cancer (KB cell)

Observing the mechanism of apoptosis through flow cytometry method using vibrant apoptosis kits

Authorized

Dr dr Abidin Widjanarko, SpPD, KHOM
Director of Human Resources and Education, Rumah Sakit Kanker Dharmais

Test Results

Correlation between Apoptotic process in: Cervical Cancer Cell (CaSki), Colon Cancer Cell (HT-29), Breast Cancer Cell (MCF-7), and Oral Cancer Cell (KB Cell) and the Administered concentration of Chang Sheuw Tian Ran Ling Yao

In Vitro Cytotoxic Study and Detection of Apoptosis through the administration of Chang Sheuw Tian Ran Ling Yao Capsule Ethanol Extract on Cancer Cells Cancer Cell Line

Results Summary

From the results of the study the IC50 value of Chang Sheuw Tian Ran Ling Yao’s ethanol extract was 50μg/ml for cervical cancer cells, 25μg/ml for colon cancer cells, 100μg/ml for breast cancer cells and, 25μg/ml for oral cancer cells after 24 hours of incubation. Correlation analysis between concentration and percentage of cancer apoptosis was 61.95% for cervical cancer cells, 63.73% for colon cancer cells 68.42% for breast cancer cells and 65.12% for oral cancer cells at α = 0.05.

These results show two things as follows: The ethanol extract of Chang Sheuw Tian Ran Ling Yao has a high cytotoxic effect on cervical cancer cells, colon cancer cells, and oral cancer cells. While possessing a high potential to influence a high rate of apoptosis in oral cancer cells, colon cancer cells, breast cancer cells, and oral cancer cells.

Conclusion

The ethanol extract of Chang Sheuw Tian Ran Ling Yao, has a high cytotoxic effect in inhibiting the growth of cervical cancer cells (CaSki line), colon cancer cells (HT-29) and oral cancer cells (KB), but has a low cytotoxic effect on breast cells (MCF-7). However, The ethanol extract of Chang Sheuw Tian Ran Ling Yao, has a high potential to affect the process of apoptosis in breast cancer cells (MCF-7) and oral cancer cells (KB cell). Hence, this study suggests that ethanol extract of Chang Sheuw Tian Ran Ling Yao has high potential of inducing high rates of cytotoxic and apoptotic activities in combating the four investigated cancer cell lines.